Reference number |
Lab-code |
Analyses |
Matrix |
|
M000 |
MVB |
Sample pre-treatment, administration In accordance with ISO 6887-1:2017 |
Food and environmental samples |
Per sample |
M003 |
Ak30_t |
Aerobic plate count 30°C in food In accordance with ISO 4833-1:2013 |
Food |
Enumeration cfu/gram |
M069 |
Ak30pf_t |
Aerobic plate count 30°C petrifilm in food Equivalent to ISO 4833-1:2013
Instead of growth medium PCA a petrifilm (3M) is used. Validated by Afnor 3M 01/1-09/89 and internally
|
Food |
Enumeration cfu/gram |
M001 |
Aes_t |
Aerobic spore formers Own method
After heating the product 10 minutes at 80°C, growth at TSA medium after 24 hours at 37°C is counted. Method commonly used and described in “Microbiologie van Voedoingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally |
Food |
Enumeration cfu/gram |
M007 |
Ans_t |
Anaerobic spore formers After heating the product 10 minutes at 80°C, anaerobic growth at SA medium after 48 hours at 37°C is counted.
Method commonly used and described in “Microbiologie van Voedoingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally. |
Food |
Enumeration cfu/gram |
M011 |
Bc_t |
Bacillus cereus In accordance with ISO 7932:2004 |
Food |
Enumeration cfu/gram |
M021 |
Cf_t |
Coliforms in foods In accordance with ISO 4832:2006 |
Food |
Enumeration cfu/gram |
M027 |
Ec_t |
E. coli In accordance with ISO 16649-2:2001 |
Food |
Enumeration cfu/gram |
M077 |
EcH7_g |
E. coli O157:H7 in meat or vegetables In accordance with AOAC 996.09:2012 |
Food |
Detection in 25 gram |
M023 |
En_t |
Enterobacteriaceae in food In accordance with ISO 21528-2:2004 without confirmation |
Food |
Enumeration cfu/gram |
M116 |
Gdroog_t Sdroog_t GSdroog_t
|
Yeasts and/or moulds in products with Water activity ≤0,95 In accordance with ISO 21527-2:2008 |
Food |
Enumeration cfu/gram |
M117 |
Gnat_t Snat_t GSnat_t |
Yeasts and/or moulds in products with Water activity >0,95 In accordance with ISO 21527-1:2008 |
Food |
Enumeration cfu/gram |
M032 |
Lb_t |
Lactobacilli enumeration in dairy products In accordance with NEN 6815:2009 |
Food |
Enumeration cfu/gram |
M033 |
Lm_g
|
Listeria monocytogenes in food and environmental samples In accordance with ISO 11290-1:2017 Validated internally for swabs |
Food and environmental samples |
Detection in 25 gram |
Lmswab_g |
Detection in swab |
|||
M033a |
Lm200_fg |
Listeria monocytogenes in food In accordance with ISO 11290-1:2017 Validated internally for filtrated samples.
|
Food |
Detection in 200 ml |
M109 |
LmPCR_g
|
Listeria monocytogenes in food and environmental samples Equivalent to ISO 11290-1:2017 Validated internally for swabs
After 24 hours incubation in 24 LEB broth at 37°C, the presence of DNA is detected by PCR. Validated by AFNOR UNI 03/08-11/13 and internally |
Food and environmental samples |
Detection in 25 gram |
LmPCRswab_g |
Detection in swab |
|||
M109a |
LmPCR200_fg |
Listeria monocytogenes in food Equivalent to ISO 11290-1:2017 Validated internally for filtrated samples.
After 24 hours incubation in 24 LEB broth at 37°C, the presence of DNA is detected by PCR. Validated by AFNOR UNI 03/08-11/13 and internally |
Food |
Detection in 200 ml |
M035 |
Lm100_t
|
Listeria monocytogenes enumeration < 100 cfu/g In accordance with ISO 11290-2:2017 |
Food |
Enumeration cfu/gram |
Lm10_t
|
Listeria monocytogenes enumeration < 10 cfu/g In accordance with ISO 11290-2:2017 |
|||
Lm1_t |
Listeria monocytogenes enumeration < 1 cfu/g In accordance with ISO 11290-2:2017 |
|||
M036 |
Mzb_t |
Lactic acid bacteria in food In accordance with ISO 15214:1998, including confirmation |
Food |
Enumeration cfu/gram |
M047 |
Salm_g
|
Salmonella spp. detection in food Equivalent to ISO 6579:2017
After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally |
Food |
Detection in 25 gram
|
Salm10_g |
Detection in 10 gram |
|||
M047b |
Salm200_fg |
Salmonella spp. detection in food Equivalent to ISO 6579:2017 Validated internally for filtrated samples.
After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally |
Food |
Detection in 200 ml |
M108 |
SalmPCR_g |
Salmonella spp. detection in food Equivalent to ISO 6579:2017
After 22 hours incubation in BPW at 37°C, the presence of DNA is detected by PCR. Validated by AFNOR UNI 03/07-11/13 and internally |
Food |
Detection in 25 gram |
M067 |
STEC25_g |
Shiga-toxin producing Escherichia coli (STEC) screening on Stx and eae genes In accordance with ISO/TS 13136:2012
Validated internally for environmental samples and filtrated samples |
Food and environmental samples |
Detection in 25 gram |
STECswab_g |
Detection in swab |
|||
STEC125_g |
Detectionin 125 gram |
|||
STEC454_g |
Detection in 454 gram |
|||
M067c |
STEC100_fg |
Detection in 100 ml |
||
|
STEC200_fg |
Detection in 200 ml |
||
M068 |
STECbev STECbev_rein |
Shiga-toxin producing Escherichia coli (STEC) confirmation In accordance with ISO/TS 13136:2012 |
Food |
Confirmation procedure on Stx en eae genes |
M083 |
STECsero |
Serotyping isolates RT-PCR O26, O45,O103, O111, O121, O145, O157 In accordance with ISO/TS 13136:2012 |
Food |
Serotypering isolaten |
M054 |
Sac100_t Sac10_t
|
Staphylococcus aureus In accordance with ISO 6888-2:1999 |
Food |
Enumeration cfu/gram |
M017 |
SredS_t |
Spores of Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213:2003 |
Food |
Enumeration cfu/gram |
|
SredB_t |
Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213:2003 |
Bijlage 13 Versie 1 D.d. 18-12-19