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Accredited methods description 2024-03-13

Accredited methods description

                                                                                                                                                               

Reference number

Lab-code

Analyses

Matrix

 

M003

Ak30_t

Aerobic plate count 30°C in food

In accordance with ISO 4833-1:2013

Food

Enumeration cfu/gram

M069

Ak30pf_t

Aerobic plate count 30°C petrifilm in food

Equivalent to ISO 4833-1:2013

 

Instead of growth medium PCA, petrifilm (3M) is used. Validated by Afnor 3M 01/1-09/89 and internally

Food

Enumeration cfu/gram

M001

Aes_t

Aerobic spore formers

Own method

 

After heating the product 10 minutes at 80°C, growth at TSA medium after 24 hours at 37°C is counted.

Method commonly used and described in “Microbiologie van Voedingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally

Food

Enumeration cfu/gram

M007

Ans_t

Anaerobic spore formers

After heating the product 10 minutes at 80°C, anaerobic growth at SA medium after 48 hours at 37°C is counted.

 

Method commonly used and described in “Microbiologie van Voed

ingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally.

Food

Enumeration cfu/gram

M011

Bc_t

Bacillus cereus

In accordance with ISO 7932:2004

Food

Enumeration cfu/gram

M021

Cf_t

Coliforms in foods

In accordance with ISO 4832:2006

Food

Enumeration cfu/gram

M201, M201a, M201b, M201c

Challengetest Listeria monocytogenes

Determination of growth potential of Listeria monocytogenes; challenge test

In accordance with ISO 20796-1:2019 and EURL Lm Technical Guidance Document, version 4, 01-07-2021

Food

-

M027

Ec_t

E. coli

In accordance with ISO 16649-2:2001

Food

Enumeration cfu/gram

M077

EcH7_g

E. coli O157:H7 in meat or vegetables

In accordance with AOAC 996.09:2012

Food

Detection in 25 gram

M023

En_t

Enterobacteriaceae in food

In accordance with ISO 21528-2:2004 without confirmation

Food

Enumeration cfu/gram

M116

Gdroog_t

Sdroog_t

GSdroog_t

 

Yeasts and/or moulds in products with Water activity ≤0,95

In accordance with ISO 21527-2:2008

Food

Enumeration cfu/gram

M117

Gnat_t

Snat_t

GSnat_t

Yeasts and/or moulds in products with Water activity >0,95

In accordance with ISO 21527-1:2008

Food

Enumeration cfu/gram

M032

Lb_t

Lactobacilli enumeration in dairy products

In accordance with NEN 6815:2009

Food

Enumeration cfu/gram

M033

Lm_g

 

Listeria monocytogenes in food and environmental samples

In accordance with ISO 11290-1:2017

Validated internally for swabs

Food and environmental samples

Detection in 25 gram

Lmswab_g

Detection in swab

M033a

Lm200_fg

Listeria monocytogenes in food

In accordance with ISO 11290-1:2017

Validated internally for filtrated samples

Food

Detection in 200 ml

M033b

LmR_g

Listeria monocytogenes in food and environmental samples

Equivalent to ISO 11290-1:2017

Food and environmental samples

Detection in 25 gram

LmRswab_g

Detection in swab

M120

LmPCR_g

 

 

Listeria monocytogenes in food and environmental samples

Equivalent to ISO 11290-1:2017

Validated by BIO 12/40-11/16 and internally

Food and environmental samples

Detection in 25 gram

LmPCRswab_g

Detection in swab

M120a

LmPCR200_fg

Listeria monocytogenes in food

Equivalent to ISO 11290-1:2017

Validated by BIO 12/40-11/16 and internally

Food and environmental samples

Detection in 200 ml

M035

Lm100_t

 

Listeria monocytogenes enumeration < 100 cfu/g

In accordance with ISO 11290-2:2017

Food

Enumeration cfu/gram

Lm10_t

 

Listeria monocytogenes enumeration < 10 cfu/g

In accordance with ISO 11290-2:2017

Lm1_t

Listeria monocytogenes enumeration < 1 cfu/g

In accordance with ISO 11290-2:2017

M036

Mzb_t

Lactic acid bacteria in food

In accordance with ISO 15214:1998, including confirmation

Food

Enumeration cfu/gram

M047

Salm_g

 

 

Salmonella spp. detection in food

Equivalent to ISO 6579:2017

 

After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C.

Validated by AFNOR UNI 03/06-12/07 and internally

Food

Detection in 25 gram

 

 

Salm10_g

Detection in 10 gram

M047b

Salm200_fg

Salmonella spp. detection in food

Equivalent to ISO 6579:2017

Validated internally for filtrated samples.

 

After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C.

Validated by AFNOR UNI 03/06-12/07 and internally

Food

Detection in 200 ml

M123/M123a

SalmISO_g

Salmonella spp. detection in food

In accordance with ISO 6579-1:2017

 

Food, environmental samples and water (process water)

Detection in 25 gram

Salm10ISO_g

Detection in 10 gram

SalswabISO

Detection in swab

Salm200ISO_fg

Detection in 200 ml

M119

SalmPCR_g

Salmonella spp. detection in food

Equivalent to ISO 6579-1:2017

Validated by BIO 12/38-06/16 and internally

Food and environmental samples

Detection in 25 gram

SalPCR_10g

Detection in 10 gram

SalmPCRswab_g

Detection in swab

M119a

SalPCR200

Salmonella spp. detection in food

Equivalent to ISO 6579-1:2017

Validated by BIO 12/38-06/16 and internally

Food and environmental samples

Detection in 200 ml

M070

STEC_ST_25g

Shiga-toxin producing Escherichia coli (STEC) screening on Stx and eae genes

Equal to ISO/TS 13136:2012 (Afnor UNI 03/13-10/20)

Raw meat

(excluding poultry),

dairy products and

fruit and

vegetables

Detection in 25 gram

M071

STECbev_ST

Shiga-toxin producing Escherichia coli (STEC) confirmation

Equal to ISO/TS 13136:2012 (Afnor UNI 03/13-10/20)

Raw meat

(excluding poultry),

dairy products and

fruit and

vegetables

Confirmation procedure on Stx and eae genes

M072

O157H7_ST_g

Escherichia coli (O157:H7) detection

Equal to ISO/TS 13136:2012 (Afnor UNI 03/13-10/20)

Raw meat

(excluding poultry),

dairy products and

fruit and

vegetables

Detection in 25 gram

M078

STECsero_ST

Detection of shiga-toxin producing

Escherichia coli (STEC) O26, O103,

O111 and O145; PCR

Equal to ISO/TS 13136:2012 (Afnor UNI 03/13-10/20)

Isolates of raw

meat (excluding

poultry), dairy

products and fruit

and vegetables

Serotypering isolaten

M054

Sac100_t

Sac10_t

Staphylococcus aureus

In accordance with ISO 6888-2:2021

Food

Enumeration cfu/gram

M017

SredS_t

Spores of Sulphite-reducing bacteria (anaerobic)

In accordance with ISO 15213: 2023 without confirmation 

Food

Enumeration cfu/gram

SredB_t

Sulphite-reducing bacteria (anaerobic)

In accordance with ISO 15213: 2023 without confirmation 

Bijlage 13                                                                     Versie 10                                                         D.d. 13-03-2024

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