Accredited methods description
|
Reference number |
Lab-code |
Analyses |
Matrix |
|
|
M003 |
Ak30_t |
Aerobic plate count 30°C in food In accordance with ISO 4833-1:2013 |
Food |
Enumeration cfu/gram |
|
M069 |
Ak30pf_t |
Aerobic plate count 30°C petrifilm in food Equivalent to ISO 4833-1:2013
Instead of growth medium PCA, petrifilm (3M) is used. Validated by Afnor 3M 01/1-09/89 and internally |
Food |
Enumeration cfu/gram |
|
M001 |
Aes_t |
Aerobic spore formers Own method
After heating the product 10 minutes at 80°C, growth at TSA medium after 24 hours at 37°C is counted. Method commonly used and described in “Microbiologie van Voedingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally |
Food |
Enumeration cfu/gram |
|
M007 |
Ans_t |
Anaerobic spore formers After heating the product 10 minutes at 80°C, anaerobic growth at SA medium after 48 hours at 37°C is counted.
Method commonly used and described in “Microbiologie van Voed ingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally. |
Food |
Enumeration cfu/gram |
|
M011 |
Bc_t |
Bacillus cereus In accordance with ISO 7932:2004 |
Food |
Enumeration cfu/gram |
|
M021 |
Cf_t |
Coliforms in foods In accordance with ISO 4832:2006 |
Food |
Enumeration cfu/gram |
|
M201, M201a, M201b, M201c |
Challengetest Listeria monocytogenes |
Determination of growth potential of Listeria monocytogenes; challenge test In accordance with ISO 20796-1:2019 and EURL Lm Technical Guidance Document, version 4, 01-07-2021 |
Food |
- |
|
M027 |
Ec_t |
E. coli In accordance with ISO 16649-2:2001 |
Food |
Enumeration cfu/gram |
|
M077 |
EcH7_g |
E. coli O157:H7 in meat or vegetables In accordance with AOAC 996.09:2012 |
Food |
Detection in 25 gram |
|
M023 |
En_t |
Enterobacteriaceae in food In accordance with ISO 21528-2:2004 without confirmation |
Food |
Enumeration cfu/gram |
|
M116 |
Gdroog_t Sdroog_t GSdroog_t
|
Yeasts and/or moulds in products with Water activity ≤0,95 In accordance with ISO 21527-2:2008 |
Food |
Enumeration cfu/gram |
|
M117 |
Gnat_t Snat_t GSnat_t |
Yeasts and/or moulds in products with Water activity >0,95 In accordance with ISO 21527-1:2008 |
Food |
Enumeration cfu/gram |
|
M032 |
Lb_t |
Lactobacilli enumeration in dairy products In accordance with NEN 6815:2009 |
Food |
Enumeration cfu/gram |
|
M033 |
Lm_g
|
Listeria monocytogenes in food and environmental samples In accordance with ISO 11290-1:2017 Validated internally for swabs |
Food and environmental samples |
Detection in 25 gram |
|
Lmswab_g |
Detection in swab |
|||
|
M033a |
Lm200_fg |
Listeria monocytogenes in food In accordance with ISO 11290-1:2017 Validated internally for filtrated samples |
Food |
Detection in 200 ml |
|
M033b |
LmR_g |
Listeria monocytogenes in food and environmental samples Equivalent to ISO 11290-1:2017 |
Food and environmental samples |
Detection in 25 gram |
|
LmRswab_g |
Detection in swab |
|||
|
M120 |
LmPCR_g
|
Listeria monocytogenes in food and environmental samples Equivalent to ISO 11290-1:2017 Validated by BIO 12/40-11/16 and internally |
Food and environmental samples |
Detection in 25 gram |
|
LmPCRswab_g |
Detection in swab |
|||
|
M120a |
LmPCR200_fg |
Listeria monocytogenes in food Equivalent to ISO 11290-1:2017 Validated by BIO 12/40-11/16 and internally |
Food and environmental samples |
Detection in 200 ml |
|
M035 |
Lm100_t
|
Listeria monocytogenes enumeration < 100 cfu/g In accordance with ISO 11290-2:2017 |
Food |
Enumeration cfu/gram |
|
Lm10_t
|
Listeria monocytogenes enumeration < 10 cfu/g In accordance with ISO 11290-2:2017 |
|||
|
Lm1_t |
Listeria monocytogenes enumeration < 1 cfu/g In accordance with ISO 11290-2:2017 |
|||
|
M036 |
Mzb_t |
Lactic acid bacteria in food In accordance with ISO 15214:1998, including confirmation |
Food |
Enumeration cfu/gram |
|
M047 |
Salm_g
|
Salmonella spp. detection in food Equivalent to ISO 6579:2017
After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally |
Food |
Detection in 25 gram
|
|
Salm10_g |
Detection in 10 gram |
|||
|
M047b |
Salm200_fg |
Salmonella spp. detection in food Equivalent to ISO 6579:2017 Validated internally for filtrated samples.
After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally |
Food |
Detection in 200 ml |
|
M123/M123a |
SalmISO_g |
Salmonella spp. detection in food In accordance with ISO 6579-1:2017
|
Food, environmental samples and water (process water) |
Detection in 25 gram |
|
Salm10ISO_g |
Detection in 10 gram |
|||
|
SalswabISO |
Detection in swab |
|||
|
Salm200ISO_fg |
Detection in 200 ml |
|||
|
M119 |
SalmPCR_g |
Salmonella spp. detection in food Equivalent to ISO 6579-1:2017 Validated by BIO 12/38-06/16 and internally |
Food and environmental samples |
Detection in 25 gram |
|
SalPCR_10g |
Detection in 10 gram |
|||
|
SalmPCRswab_g |
Detection in swab |
|||
|
M119a |
SalPCR200 |
Salmonella spp. detection in food Equivalent to ISO 6579-1:2017 Validated by BIO 12/38-06/16 and internally |
Food and environmental samples |
Detection in 200 ml |
|
M070 |
STEC_ST_25g |
Shiga-toxin producing Escherichia coli (STEC) screening on Stx and eae genes Equal to ISO/TS 13136:2012 (Afnor UNI 03/13-10/20) |
Raw meat (excluding poultry), dairy products and fruit and vegetables |
Detection in 25 gram |
|
M071 |
STECbev_ST |
Shiga-toxin producing Escherichia coli (STEC) confirmation Equal to ISO/TS 13136:2012 (Afnor UNI 03/13-10/20) |
Raw meat (excluding poultry), dairy products and fruit and vegetables |
Confirmation procedure on Stx and eae genes |
|
M072 |
O157H7_ST_g |
Escherichia coli (O157:H7) detection Equal to ISO/TS 13136:2012 (Afnor UNI 03/13-10/20) |
Raw meat (excluding poultry), dairy products and fruit and vegetables |
Detection in 25 gram |
|
M078 |
STECsero_ST |
Detection of shiga-toxin producing Escherichia coli (STEC) O26, O103, O111 and O145; PCR Equal to ISO/TS 13136:2012 (Afnor UNI 03/13-10/20) |
Isolates of raw meat (excluding poultry), dairy products and fruit and vegetables |
Serotypering isolaten |
|
M054 |
Sac100_t Sac10_t |
Staphylococcus aureus In accordance with ISO 6888-2:2021 |
Food |
Enumeration cfu/gram |
|
M017 |
SredS_t |
Spores of Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213: 2023 without confirmation |
Food |
Enumeration cfu/gram |
|
SredB_t |
Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213: 2023 without confirmation |
Bijlage 13 Versie 10 D.d. 13-03-2024