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Accredited methods description

Reference number	Lab-code	Analyses	Matrix
M003	Ak30_t	Aerobic plate count 30°C in food In accordance with ISO 4833-1:2013	Food	Enumeration cfu/gram
M069	Ak30pf_t	Aerobic plate count 30°C petrifilm in food Equivalent to ISO 4833-1:2013 Instead of growth medium PCA, petrifilm (3M) is used. Validated by Afnor 3M 01/1-09/89 and internally	Food	Enumeration cfu/gram
M001	Aes_t	Aerobic spore formers Own method After heating the product 10 minutes at 80°C, growth at TSA medium after 24 hours at 37°C is counted. Method commonly used and described in “Microbiologie van Voedingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally	Food	Enumeration cfu/gram
M007	Ans_t	Anaerobic spore formers After heating the product 10 minutes at 80°C, anaerobic growth at SA medium after 48 hours at 37°C is counted. Method commonly used and described in “Microbiologie van Voedingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally.	Food	Enumeration cfu/gram
M011	Bc_t	Bacillus cereus In accordance with ISO 7932:2004	Food	Enumeration cfu/gram
M021	Cf_t	Coliforms in foods In accordance with ISO 4832:2006	Food	Enumeration cfu/gram
M027	Ec_t	E. coli In accordance with ISO 16649-2:2001	Food	Enumeration cfu/gram
M077	EcH7_g	E. coli O157:H7 in meat or vegetables In accordance with AOAC 996.09:2012	Food	Detection in 25 gram
M023	En_t	Enterobacteriaceae in food In accordance with ISO 21528-2:2004 without confirmation	Food	Enumeration cfu/gram
M116	Gdroog_t Sdroog_t GSdroog_t	Yeasts and/or moulds in products with Water activity ≤0,95 In accordance with ISO 21527-2:2008	Food	Enumeration cfu/gram
M117	Gnat_t Snat_t GSnat_t	Yeasts and/or moulds in products with Water activity >0,95 In accordance with ISO 21527-1:2008	Food	Enumeration cfu/gram
M032	Lb_t	Lactobacilli enumeration in dairy products In accordance with NEN 6815:2009	Food	Enumeration cfu/gram
M033	Lm_g	Listeria monocytogenes in food and environmental samples In accordance with ISO 11290-1:2017 Validated internally for swabs	Food and environmental samples	Detection in 25 gram
M033	Lmswab_g		Food and environmental samples	Detection in swab
M033a	Lm200_fg	Listeria monocytogenes in food In accordance with ISO 11290-1:2017 Validated internally for filtrated samples	Food	Detection in 200 ml
M033b	LmR_g	Listeria monocytogenes in food and environmental samples Equivalent to ISO 11290-1:2017	Food and environmental samples	Detection in 25 gram
M033b	LmRswab_g		Food and environmental samples	Detection in swab
M109/ M120	LmPCR_g	Listeria monocytogenes in food and environmental samples Equivalent to ISO 11290-1:2017 Validated by AFNOR UNI 03/08-11/13 / BIO 12/40-11/16 and internally	Food and environmental samples	Detection in 25 gram
M109/ M120	LmPCRswab_g		Food and environmental samples	Detection in swab
M109a/ M120a	LmPCR200_fg	Listeria monocytogenes in food Equivalent to ISO 11290-1:2017 Validated by AFNOR UNI 03/08-11/13 / BIO 12/40-11/16 and internally	Food and environmental samples	Detection in 200 ml
M035	Lm100_t	Listeria monocytogenes enumeration < 100 cfu/g In accordance with ISO 11290-2:2017	Food	Enumeration cfu/gram
	Lm10_t	Listeria monocytogenes enumeration < 10 cfu/g In accordance with ISO 11290-2:2017
	Lm1_t	Listeria monocytogenes enumeration < 1 cfu/g In accordance with ISO 11290-2:2017
M036	Mzb_t	Lactic acid bacteria in food In accordance with ISO 15214:1998, including confirmation	Food	Enumeration cfu/gram
M047	Salm_g	Salmonella spp. detection in food Equivalent to ISO 6579:2017 After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally	Food	Detection in 25 gram
M047	Salm10_g		Food	Detection in 10 gram
M047b	Salm200_fg	Salmonella spp. detection in food Equivalent to ISO 6579:2017 Validated internally for filtrated samples. After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally	Food	Detection in 200 ml
M108/ M119	SalmPCR_g	Salmonella spp. detection in food Equivalent to ISO 6579-1:2017 Validated by AFNOR UNI 03/07-11/13 / BIO 12/38-06/16 and internally	Food and environmental samples	Detection in 25 gram
M108/ M119	SalmPCRswab_g		Food and environmental samples	Detection in swab
M108a/M119a	SalPCR200	Salmonella spp. detection in food Equivalent to ISO 6579-1:2017 Validated by AFNOR UNI 03/07-11/13 / BIO 12/38-06/16 and internally	Food and environmental samples	Detection in 200 ml
M067	STEC25_g	Shiga-toxin producing Escherichia coli (STEC) screening on Stx and eae genes In accordance with ISO/TS 13136:2012 Validated internally for environmental samples and filtrated samples	Food and environmental samples	Detection in 25 gram
	STECswab_g			Detection in swab
	STEC125_g			Detection in 125 gram
	STEC454_g			Detection in 454 gram
M067c	STEC100_fg			Detection in 100 ml
	STEC200_fg			Detection in 200 ml
M068	STECbev STECbev_rein	Shiga-toxin producing Escherichia coli (STEC) confirmation In accordance with ISO/TS 13136:2012	Food	Confirmation procedure on Stx and eae genes
M083	STECsero	Serotyping of isolates by RT-PCR O26, O45, O103, O111, O121, O145, O157 In accordance with ISO/TS 13136:2012	Food	Serotypering isolaten
M054	Sac100_t Sac10_t	Staphylococcus aureus In accordance with ISO 6888-2:1999	Food	Enumeration cfu/gram
M017	SredS_t	Spores of Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213:2003	Food	Enumeration cfu/gram
M017	SredB_t	Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213:2003	Food	Enumeration cfu/gram
M061	Water	Escherichia coli and coliforms In accordance with ISO 9308-1:2014	Water (process water)	Detection in 100 ml

The method descriptions of each accredited method under supervision of the Dutch council for Accreditation are listed below

Reference number	Lab-code	Analyses	Matrix	Units
M003	Ak30_t	Aerobic plate count 30°C in food In accordance with ISO 4833-1:2013	Food	Enumeration cfu/gram
M069	Ak30pf_t	Aerobic plate count 30°C petrifilm in food Equivalent to ISO 4833-1:2013 Instead of growth medium PCA, petrifilm (3M) is used. Validated by Afnor 3M 01/1-09/89 and internally	Food	Enumeration cfu/gram
M001	Aes_t	Aerobic spore formers Own method After heating the product 10 minutes at 80°C, growth at TSA medium after 24 hours at 37°C is counted. Method commonly used and described in “Microbiologie van Voedingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally	Food	Enumeration cfu/gram
M007	Ans_t	Anaerobic spore formers After heating the product 10 minutes at 80°C, anaerobic growth at SA medium after 48 hours at 37°C is counted. Method commonly used and described in “Microbiologie van Voedings-middelen”, ISBN 978-90-8572-056-0. Validated internally.	Food	Enumeration cfu/gram
M011	Bc_t	Bacillus cereus In accordance with ISO 7932:2004	Food	Enumeration cfu/gram
M021	Cf_t	Coliforms in foods In accordance with ISO 4832:2006	Food	Enumeration cfu/gram
M027	Ec_t	E. coli In accordance with ISO 16649-2:2001	Food	Enumeration cfu/gram
M077	EcH7_g	E. coli O157:H7 in meat or vegetables In accordance with AOAC 996.09:2012	Food	Detection in 25 gram
M023	En_t	Enterobacteriaceae in food In accordance with ISO 21528-2:2004 without confirmation	Food	Enumeration cfu/gram
M116	Gdroog_t Sdroog_t GSdroog_t	Yeasts and/or moulds in products with Water activity ≤0,95 In accordance with ISO 21527-2:2008	Food	Enumeration cfu/gram
M117	Gnat_t Snat_t GSnat_t	Yeasts and/or moulds in products with Water activity >0,95 In accordance with ISO 21527-1:2008	Food	Enumeration cfu/gram
M032	Lb_t	Lactobacilli enumeration in dairy products In accordance with NEN 6815:2009	Food	Enumeration cfu/gram
M033	Lm_g	Listeria monocytogenes in food and environmental samples In accordance with ISO 11290-1:2017 Validated internally for swabs	Food and environmental samples	Detection in 25 gram
M033	Lmswab_g		Food and environmental samples	Detection in swab
M033a	Lm200_fg	Listeria monocytogenes in food In accordance with ISO 11290-1:2017 Validated internally for filtrated samples.	Food	Detection in 200 ml
M033b	LmR_g	Listeria monocytogenes in food and environmental samples Equivalent to ISO 11290-1:2017	Food and environmental samples	Detection in 25 gram
M033b	LmRswab_g		Food and environmental samples	Detection in swab
M109	LmPCR_g	Listeria monocytogenes in food and environmental samples Equivalent to ISO 11290-1:2017 Validated internally for swabs After 24 hours incubation in 24 LEB broth at 37°C, the presence of DNA is detected by PCR. Validated by AFNOR UNI 03/08-11/13 and internally	Food and environmental samples	Detection in 25 gram
M109	LmPCRswab_g		Food and environmental samples	Detection in swab
M109a	LmPCR200_fg	Listeria monocytogenes in food Equivalent to ISO 11290-1:2017 Validated internally for filtrated samples. After 24 hours incubation in 24 LEB broth at 37°C, the presence of DNA is detected by PCR. Validated by AFNOR UNI 03/08-11/13 and internally	Food	Detection in 200 ml
M035	Lm100_t	Listeria monocytogenes enumeration < 100 cfu/g In accordance with ISO 11290-2:2017	Food	Enumeration cfu/gram
	Lm10_t	Listeria monocytogenes enumeration < 10 cfu/g In accordance with ISO 11290-2:2017
	Lm1_t	Listeria monocytogenes enumeration < 1 cfu/g In accordance with ISO 11290-2:2017
M036	Mzb_t	Lactic acid bacteria in food In accordance with ISO 15214:1998, including confirmation	Food	Enumeration cfu/gram
M047	Salm_g	Salmonella spp. detection in food Equivalent to ISO 6579:2017 After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally	Food	Detection in 25 gram
M047	Salm10_g		Food	Detection in 10 gram
M047b	Salm200_fg	Salmonella spp. detection in food Equivalent to ISO 6579:2017 Validated internally for filtrated samples. After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally	Food	Detection in 200 ml
M108	SalmPCR_g	Salmonella spp. detection in food Equivalent to ISO 6579:2017 After 22 hours incubation in BPW at 37°C, the presence of DNA is detected by PCR. Validated by AFNOR UNI 03/07-11/13 and internally	Food	Detection in 25 gram
M067	STEC25_g	Shiga-toxin producing Escherichia coli (STEC) screening on Stx and eae genes In accordance with ISO/TS 13136:2012 Validated internally for environmental samples and filtrated samples	Food and environmental samples	Detection in 25 gram
	STECswab_g			Detection in swab
	STEC125_g			Detection in 125 gram
	STEC454_g			Detection in 454 gram
M067c	STEC100_fg			Detection in 100 ml
	STEC200_fg			Detection in 200 ml
M068	STECbev STECbev_rein	Shiga-toxin producing Escherichia coli (STEC) confirmation In accordance with ISO/TS 13136:2012	Food	Confirmation procedure on Stx and eae genes
M083	STECsero	Serotyping of isolates by RT-PCR O26, O45, O103, O111, O121, O145, O157 In accordance with ISO/TS 13136:2012	Food	Serotypering isolates
M054	Sac100_t Sac10_t	Staphylococcus aureus In accordance with ISO 6888-2:1999	Food	Enumeration cfu/gram
M017	SredS_t	Spores of Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213:2003	Food	Enumeration cfu/gram
M017	SredB_t	Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213:2003	Food	Enumeration cfu/gram
M061	Water	Escherichia coli and coliforms In accordance with ISO 9308-1:2014	Water (process water)	Detection in 100 ml

Reference number	Lab-code	Analyses	Matrix
M000	MVB	Sample pre-treatment, administration In accordance with ISO 6887-1:2017	Food and environmental samples	Per sample
M003	Ak30_t	Aerobic plate count 30°C in food In accordance with ISO 4833-1:2013	Food	Enumeration cfu/gram
M069	Ak30pf_t	Aerobic plate count 30°C petrifilm in food Equivalent to ISO 4833-1:2013 Instead of growth medium PCA a petrifilm (3M) is used. Validated by Afnor 3M 01/1-09/89 and internally	Food	Enumeration cfu/gram
M001	Aes_t	Aerobic spore formers Own method After heating the product 10 minutes at 80°C, growth at TSA medium after 24 hours at 37°C is counted. Method commonly used and described in “Microbiologie van Voedoingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally	Food	Enumeration cfu/gram
M007	Ans_t	Anaerobic spore formers After heating the product 10 minutes at 80°C, anaerobic growth at SA medium after 48 hours at 37°C is counted. Method commonly used and described in “Microbiologie van Voedoingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally.	Food	Enumeration cfu/gram
M011	Bc_t	Bacillus cereus In accordance with ISO 7932:2004	Food	Enumeration cfu/gram
M021	Cf_t	Coliforms in foods In accordance with ISO 4832:2006	Food	Enumeration cfu/gram
M027	Ec_t	E. coli In accordance with ISO 16649-2:2001	Food	Enumeration cfu/gram
M077	EcH7_g	E. coli O157:H7 in meat or vegetables In accordance with AOAC 996.09:2012	Food	Detection in 25 gram
M023	En_t	Enterobacteriaceae in food In accordance with ISO 21528-2:2004 without confirmation	Food	Enumeration cfu/gram
M116	Gdroog_t Sdroog_t GSdroog_t	Yeasts and/or moulds in products with Water activity ≤0,95 In accordance with ISO 21527-2:2008	Food	Enumeration cfu/gram
M117	Gnat_t Snat_t GSnat_t	Yeasts and/or moulds in products with Water activity >0,95 In accordance with ISO 21527-1:2008	Food	Enumeration cfu/gram
M032	Lb_t	Lactobacilli enumeration in dairy products In accordance with NEN 6815:2009	Food	Enumeration cfu/gram
M033	Lm_g	Listeria monocytogenes in food and environmental samples In accordance with ISO 11290-1:2017 Validated internally for swabs	Food and environmental samples	Detection in 25 gram
M033	Lmswab_g		Food and environmental samples	Detection in swab
M033a	Lm200_fg	Listeria monocytogenes in food In accordance with ISO 11290-1:2017 Validated internally for filtrated samples.	Food	Detection in 200 ml
M109	LmPCR_g	Listeria monocytogenes in food and environmental samples Equivalent to ISO 11290-1:2017 Validated internally for swabs After 24 hours incubation in 24 LEB broth at 37°C, the presence of DNA is detected by PCR. Validated by AFNOR UNI 03/08-11/13 and internally	Food and environmental samples	Detection in 25 gram
M109	LmPCRswab_g		Food and environmental samples	Detection in swab
M109a	LmPCR200_fg	Listeria monocytogenes in food Equivalent to ISO 11290-1:2017 Validated internally for filtrated samples. After 24 hours incubation in 24 LEB broth at 37°C, the presence of DNA is detected by PCR. Validated by AFNOR UNI 03/08-11/13 and internally	Food	Detection in 200 ml
M035	Lm100_t	Listeria monocytogenes enumeration < 100 cfu/g In accordance with ISO 11290-2:2017	Food	Enumeration cfu/gram
	Lm10_t	Listeria monocytogenes enumeration < 10 cfu/g In accordance with ISO 11290-2:2017
	Lm1_t	Listeria monocytogenes enumeration < 1 cfu/g In accordance with ISO 11290-2:2017
M036	Mzb_t	Lactic acid bacteria in food In accordance with ISO 15214:1998, including confirmation	Food	Enumeration cfu/gram
M047	Salm_g	Salmonella spp. detection in food Equivalent to ISO 6579:2017 After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally	Food	Detection in 25 gram
M047	Salm10_g		Food	Detection in 10 gram
M047b	Salm200_fg	Salmonella spp. detection in food Equivalent to ISO 6579:2017 Validated internally for filtrated samples. After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally	Food	Detection in 200 ml
M108	SalmPCR_g	Salmonella spp. detection in food Equivalent to ISO 6579:2017 After 22 hours incubation in BPW at 37°C, the presence of DNA is detected by PCR. Validated by AFNOR UNI 03/07-11/13 and internally	Food	Detection in 25 gram
M067	STEC25_g	Shiga-toxin producing Escherichia coli (STEC) screening on Stx and eae genes In accordance with ISO/TS 13136:2012 Validated internally for environmental samples and filtrated samples	Food and environmental samples	Detection in 25 gram
	STECswab_g			Detection in swab
	STEC125_g			Detectionin 125 gram
	STEC454_g			Detection in 454 gram
M067c	STEC100_fg			Detection in 100 ml
	STEC200_fg			Detection in 200 ml
M068	STECbev STECbev_rein	Shiga-toxin producing Escherichia coli (STEC) confirmation In accordance with ISO/TS 13136:2012	Food	Confirmation procedure on Stx en eae genes
M083	STECsero	Serotyping isolates RT-PCR O26, O45,O103, O111, O121, O145, O157 In accordance with ISO/TS 13136:2012	Food	Serotypering isolaten
M054	Sac100_t Sac10_t	Staphylococcus aureus In accordance with ISO 6888-2:1999	Food	Enumeration cfu/gram
M017	SredS_t	Spores of Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213:2003	Food	Enumeration cfu/gram
	SredB_t	Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213:2003	Food	Enumeration cfu/gram

Bijlage 13 Versie 1 D.d. 18-12-19

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