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Microbiologie

Informatie over de microbiologie

Reference number	Lab-code	Analyses	Matrix
M003	Ak30_t	Aerobic plate count 30°C in food In accordance with ISO 4833-1:2013	Food	Enumeration cfu/gram
M069	Ak30pf_t	Aerobic plate count 30°C petrifilm in food Equivalent to ISO 4833-1:2013 Instead of growth medium PCA, petrifilm (3M) is used. Validated by Afnor 3M 01/1-09/89 and internally	Food	Enumeration cfu/gram
M001	Aes_t	Aerobic spore formers Own method After heating the product 10 minutes at 80°C, growth at TSA medium after 24 hours at 37°C is counted. Method commonly used and described in “Microbiologie van Voedingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally	Food	Enumeration cfu/gram
M007	Ans_t	Anaerobic spore formers After heating the product 10 minutes at 80°C, anaerobic growth at SA medium after 48 hours at 37°C is counted. Method commonly used and described in “Microbiologie van Voed ingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally.	Food	Enumeration cfu/gram
M011	Bc_t	Bacillus cereus In accordance with ISO 7932:2004	Food	Enumeration cfu/gram
M021	Cf_t	Coliforms in foods In accordance with ISO 4832:2006	Food	Enumeration cfu/gram
M201, M201a, M201b, M201c	Challengetest Listeria monocytogenes	Determination of growth potential of Listeria monocytogenes; challenge test *In accordance with ISO 20796-1:2019 and EURL Lm Technical Guidance Document, version 4, 01-07-2021*	Food	-
M027	Ec_t	E. coli In accordance with ISO 16649-2:2001	Food	Enumeration cfu/gram
M077	EcH7_g	E. coli O157:H7 in meat or vegetables In accordance with AOAC 996.09:2012	Food	Detection in 25 gram
M023	En_t	Enterobacteriaceae in food In accordance with ISO 21528-2:2004 without confirmation	Food	Enumeration cfu/gram
M116	Gdroog_t Sdroog_t GSdroog_t	Yeasts and/or moulds in products with Water activity ≤0,95 In accordance with ISO 21527-2:2008	Food	Enumeration cfu/gram
M117	Gnat_t Snat_t GSnat_t	Yeasts and/or moulds in products with Water activity >0,95 In accordance with ISO 21527-1:2008	Food	Enumeration cfu/gram
M032	Lb_t	Lactobacilli enumeration in dairy products In accordance with NEN 6815:2009	Food	Enumeration cfu/gram
M033	Lm_g	Listeria monocytogenes in food and environmental samples In accordance with ISO 11290-1:2017 Validated internally for swabs	Food and environmental samples	Detection in 25 gram
M033	Lmswab_g		Food and environmental samples	Detection in swab
M033a	Lm200_fg	Listeria monocytogenes in food In accordance with ISO 11290-1:2017 Validated internally for filtrated samples	Food	Detection in 200 ml
M033b	LmR_g	Listeria monocytogenes in food and environmental samples Equivalent to ISO 11290-1:2017	Food and environmental samples	Detection in 25 gram
M033b	LmRswab_g		Food and environmental samples	Detection in swab
M120	LmPCR_g	Listeria monocytogenes in food and environmental samples Equivalent to ISO 11290-1:2017 Validated by BIO 12/40-11/16 and internally	Food and environmental samples	Detection in 25 gram
M120	LmPCRswab_g		Food and environmental samples	Detection in swab
M120a	LmPCR200_fg	Listeria monocytogenes in food Equivalent to ISO 11290-1:2017 Validated by BIO 12/40-11/16 and internally	Food and environmental samples	Detection in 200 ml
M035	Lm100_t	Listeria monocytogenes enumeration < 100 cfu/g In accordance with ISO 11290-2:2017	Food	Enumeration cfu/gram
	Lm10_t	Listeria monocytogenes enumeration < 10 cfu/g In accordance with ISO 11290-2:2017
	Lm1_t	Listeria monocytogenes enumeration < 1 cfu/g In accordance with ISO 11290-2:2017
M036	Mzb_t	Lactic acid bacteria in food In accordance with ISO 15214:1998, including confirmation	Food	Enumeration cfu/gram
M047	Salm_g	Salmonella spp. detection in food Equivalent to ISO 6579:2017 After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally	Food	Detection in 25 gram
M047	Salm10_g		Food	Detection in 10 gram
M047b	Salm200_fg	Salmonella spp. detection in food Equivalent to ISO 6579:2017 Validated internally for filtrated samples. After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally	Food	Detection in 200 ml
M123/M123a	SalmISO_g	Salmonella spp. detection in food In accordance with ISO 6579-1:2017	Food, environmental samples and water (process water)	Detection in 25 gram
	Salm10ISO_g			Detection in 10 gram
	SalswabISO			Detection in swab
	Salm200ISO_fg			Detection in 200 ml
M119	SalmPCR_g	Salmonella spp. detection in food Equivalent to ISO 6579-1:2017 Validated by BIO 12/38-06/16 and internally	Food and environmental samples	Detection in 25 gram
	SalPCR_10g			Detection in 10 gram
	SalmPCRswab_g			Detection in swab
M119a	SalPCR200	Salmonella spp. detection in food Equivalent to ISO 6579-1:2017 Validated by BIO 12/38-06/16 and internally	Food and environmental samples	Detection in 200 ml
M067	STEC25_g	Shiga-toxin producing Escherichia coli (STEC) screening on Stx and eae genes In accordance with ISO/TS 13136:2012 Validated internally for environmental samples and filtrated samples	Food and environmental samples	Detection in 25 gram
	STECswab_g			Detection in swab
	STEC125_g			Detection in 125 gram
	STEC454_g			Detection in 454 gram
M067c	STEC100_fg			Detection in 100 ml
	STEC200_fg			Detection in 200 ml
M068	STECbev STECbev_rein	Shiga-toxin producing Escherichia coli (STEC) confirmation In accordance with ISO/TS 13136:2012	Food	Confirmation procedure on Stx and eae genes
M083	STECsero	Serotyping of isolates by RT-PCR O26, O103, O111, O145, O157 In accordance with ISO/TS 13136:2012	Food	Serotypering isolaten
M054	Sac100_t Sac10_t	Staphylococcus aureus In accordance with ISO 6888-2:2021	Food	Enumeration cfu/gram
M017	SredS_t	Spores of Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213:2003	Food	Enumeration cfu/gram
M017	SredB_t	Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213:2003	Food	Enumeration cfu/gram
M061	Water	Escherichia coli and coliforms In accordance with ISO 9308-1:2014	Water (process water)	Detection in 100 ml

Bijlage 13 Versie 7 D.d. 11-02-2022

Bold = changed

Reference number	Lab-code	Analyses	Matrix
M003	Ak30_t	Aerobic plate count 30°C in food In accordance with ISO 4833-1:2013	Food	Enumeration cfu/gram
M069	Ak30pf_t	Aerobic plate count 30°C petrifilm in food Equivalent to ISO 4833-1:2013 Instead of growth medium PCA, petrifilm (3M) is used. Validated by Afnor 3M 01/1-09/89 and internally	Food	Enumeration cfu/gram
M001	Aes_t	Aerobic spore formers Own method After heating the product 10 minutes at 80°C, growth at TSA medium after 24 hours at 37°C is counted. Method commonly used and described in “Microbiologie van Voedingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally	Food	Enumeration cfu/gram
M007	Ans_t	Anaerobic spore formers After heating the product 10 minutes at 80°C, anaerobic growth at SA medium after 48 hours at 37°C is counted. Method commonly used and described in “Microbiologie van Voed ingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally.	Food	Enumeration cfu/gram
M011	Bc_t	Bacillus cereus In accordance with ISO 7932:2004	Food	Enumeration cfu/gram
M021	Cf_t	Coliforms in foods In accordance with ISO 4832:2006	Food	Enumeration cfu/gram
M027	Ec_t	E. coli In accordance with ISO 16649-2:2001	Food	Enumeration cfu/gram
M077	EcH7_g	E. coli O157:H7 in meat or vegetables In accordance with AOAC 996.09:2012	Food	Detection in 25 gram
M023	En_t	Enterobacteriaceae in food In accordance with ISO 21528-2:2004 without confirmation	Food	Enumeration cfu/gram
M116	Gdroog_t Sdroog_t GSdroog_t	Yeasts and/or moulds in products with Water activity ≤0,95 In accordance with ISO 21527-2:2008	Food	Enumeration cfu/gram
M117	Gnat_t Snat_t GSnat_t	Yeasts and/or moulds in products with Water activity >0,95 In accordance with ISO 21527-1:2008	Food	Enumeration cfu/gram
M032	Lb_t	Lactobacilli enumeration in dairy products In accordance with NEN 6815:2009	Food	Enumeration cfu/gram
M033	Lm_g	Listeria monocytogenes in food and environmental samples In accordance with ISO 11290-1:2017 Validated internally for swabs	Food and environmental samples	Detection in 25 gram
M033	Lmswab_g		Food and environmental samples	Detection in swab
M033a	Lm200_fg	Listeria monocytogenes in food In accordance with ISO 11290-1:2017 Validated internally for filtrated samples	Food	Detection in 200 ml
M033b	LmR_g	Listeria monocytogenes in food and environmental samples Equivalent to ISO 11290-1:2017	Food and environmental samples	Detection in 25 gram
M033b	LmRswab_g		Food and environmental samples	Detection in swab
M120	LmPCR_g	Listeria monocytogenes in food and environmental samples Equivalent to ISO 11290-1:2017 Validated by BIO 12/40-11/16 and internally	Food and environmental samples	Detection in 25 gram
M120	LmPCRswab_g		Food and environmental samples	Detection in swab
M120a	LmPCR200_fg	Listeria monocytogenes in food Equivalent to ISO 11290-1:2017 Validated by BIO 12/40-11/16 and internally	Food and environmental samples	Detection in 200 ml
M035	Lm100_t	Listeria monocytogenes enumeration < 100 cfu/g In accordance with ISO 11290-2:2017	Food	Enumeration cfu/gram
	Lm10_t	Listeria monocytogenes enumeration < 10 cfu/g In accordance with ISO 11290-2:2017
	Lm1_t	Listeria monocytogenes enumeration < 1 cfu/g In accordance with ISO 11290-2:2017
M036	Mzb_t	Lactic acid bacteria in food In accordance with ISO 15214:1998, including confirmation	Food	Enumeration cfu/gram
M047	Salm_g	Salmonella spp. detection in food Equivalent to ISO 6579:2017 After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally	Food	Detection in 25 gram
M047	Salm10_g		Food	Detection in 10 gram
M047b	Salm200_fg	Salmonella spp. detection in food Equivalent to ISO 6579:2017 Validated internally for filtrated samples. After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C. Validated by AFNOR UNI 03/06-12/07 and internally	Food	Detection in 200 ml
M123/M123a	SalmISO_g	Salmonella spp. detection in food In accordance with ISO 6579-1:2017	Food, environmental samples and water (process water)	Detection in 25 gram
	Salm10ISO_g			Detection in 10 gram
	SalswabISO			Detection in swab
	Salm200ISO_fg			Detection in 200 ml
M119	SalmPCR_g	Salmonella spp. detection in food Equivalent to ISO 6579-1:2017 Validated by BIO 12/38-06/16 and internally	Food and environmental samples	Detection in 25 gram
	SalPCR_10g			Detection in 10 gram
	SalmPCRswab_g			Detection in swab
M119a	SalPCR200	Salmonella spp. detection in food Equivalent to ISO 6579-1:2017 Validated by BIO 12/38-06/16 and internally	Food and environmental samples	Detection in 200 ml
M067	STEC25_g	Shiga-toxin producing Escherichia coli (STEC) screening on Stx and eae genes In accordance with ISO/TS 13136:2012 Validated internally for environmental samples and filtrated samples	Food and environmental samples	Detection in 25 gram
	STECswab_g			Detection in swab
	STEC125_g			Detection in 125 gram
	STEC454_g			Detection in 454 gram
M067c	STEC100_fg			Detection in 100 ml
	STEC200_fg			Detection in 200 ml
M068	STECbev STECbev_rein	Shiga-toxin producing Escherichia coli (STEC) confirmation In accordance with ISO/TS 13136:2012	Food	Confirmation procedure on Stx and eae genes
M083	STECsero	Serotyping of isolates by RT-PCR O26, O45, O103, O111, O121, O145, O157 In accordance with ISO/TS 13136:2012	Food	Serotypering isolaten
M054	Sac100_t Sac10_t	Staphylococcus aureus In accordance with ISO 6888-2:2021	Food	Enumeration cfu/gram
M017	SredS_t	Spores of Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213:2003	Food	Enumeration cfu/gram
M017	SredB_t	Sulphite-reducing bacteria (anaerobic) In accordance with ISO 15213:2003	Food	Enumeration cfu/gram
M061	Water	Escherichia coli and coliforms In accordance with ISO 9308-1:2014	Water (process water)	Detection in 100 ml

Bijlage 13 Versie 6 D.d. 20-12-2021

Er zijn veel traditionele manieren om microorganismen te identificeren. Er is echter een nieuwe fase is aangebroken in de microbiologie, identificatie op basis van DNR/RNA. Bij WFC hebben we gekozen om identificaties door middel van Whole Genome Sequencing in eigen beheer uit te voeren. In het verleden was deze methode, duur en tijdrovend. Nu echter zijn we in staat om binnen 48-72 uur een goede identificatie op basis van de genomen van bacterien uit te kunnen voeren voor een concurerende prijs. Een snelle en volledigere identificatie van soorten Salmonella en Listeria behoren hiermee tot de mogelijkheden. Ook zijn identificaties van andere soorten eenvoudiger en breder toegankelijk geworden.

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